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A. Representative dot plots and quantification of CD44 and CD62L expression in CD8 + T cells from the indicated non lymphoid organs in conditional SUV39H1 KO (Cre+) or WT (Cre-) littermates. B. t-SNE plots of merged flow cytometry data gated on total CD8 + T cells from the liver of 5 conditional SUV39H1 KO (Cre+) and 5 WT littermates (Cre-) at steady state. See Supp Table 1. C. Representative dot plots and quantification of <t>CD69</t> + CD103 - and CD49d + Ly6c - populations in CD44 + CD8 + T cells from liver of conditional SUV39H1 KO mice (Cre+) or WT littermates (Cre-) at steady state. D. Representative dot plots and quantification of intravascular staining negative (iv-) cells among lung CD8 + T cells and CD69 + CD103 - or CD49d + Ly6c - populations in iv-CD44 + CD8 + T cells from conditional SUV39H1 KO mice (Cre+) or WT littermates (Cre-) at steady state. Each dot represents a mouse, different shapes represent different experiments. * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by multiple unpaired t test with Holm-Šídák correction ( A and B ) or Mann Whitney test ( C and D ).
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Image Search Results


A. Representative dot plots and quantification of CD44 and CD62L expression in CD8 + T cells from the indicated non lymphoid organs in conditional SUV39H1 KO (Cre+) or WT (Cre-) littermates. B. t-SNE plots of merged flow cytometry data gated on total CD8 + T cells from the liver of 5 conditional SUV39H1 KO (Cre+) and 5 WT littermates (Cre-) at steady state. See Supp Table 1. C. Representative dot plots and quantification of CD69 + CD103 - and CD49d + Ly6c - populations in CD44 + CD8 + T cells from liver of conditional SUV39H1 KO mice (Cre+) or WT littermates (Cre-) at steady state. D. Representative dot plots and quantification of intravascular staining negative (iv-) cells among lung CD8 + T cells and CD69 + CD103 - or CD49d + Ly6c - populations in iv-CD44 + CD8 + T cells from conditional SUV39H1 KO mice (Cre+) or WT littermates (Cre-) at steady state. Each dot represents a mouse, different shapes represent different experiments. * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by multiple unpaired t test with Holm-Šídák correction ( A and B ) or Mann Whitney test ( C and D ).

Journal: bioRxiv

Article Title: Epigenetic control of CD8 + T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

doi: 10.1101/2024.05.13.593994

Figure Lengend Snippet: A. Representative dot plots and quantification of CD44 and CD62L expression in CD8 + T cells from the indicated non lymphoid organs in conditional SUV39H1 KO (Cre+) or WT (Cre-) littermates. B. t-SNE plots of merged flow cytometry data gated on total CD8 + T cells from the liver of 5 conditional SUV39H1 KO (Cre+) and 5 WT littermates (Cre-) at steady state. See Supp Table 1. C. Representative dot plots and quantification of CD69 + CD103 - and CD49d + Ly6c - populations in CD44 + CD8 + T cells from liver of conditional SUV39H1 KO mice (Cre+) or WT littermates (Cre-) at steady state. D. Representative dot plots and quantification of intravascular staining negative (iv-) cells among lung CD8 + T cells and CD69 + CD103 - or CD49d + Ly6c - populations in iv-CD44 + CD8 + T cells from conditional SUV39H1 KO mice (Cre+) or WT littermates (Cre-) at steady state. Each dot represents a mouse, different shapes represent different experiments. * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by multiple unpaired t test with Holm-Šídák correction ( A and B ) or Mann Whitney test ( C and D ).

Article Snippet: Cell suspensions were prepared in PBS and stained with Near IR (Fisher Scientific L34975) or Acqua LIVE/DEAD™ Fixable Cell Stain Kit (Fisher Scientific L34957) according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3, eBiosciences), FITC anti-CD8α (53-6.7, BD), PerCP-Cy5.5 CD8 α (53-6.7, BD), PE CD8 α Monoclonal Antibody (53-6.7, Ficher Scientific), BUV395 Rat Anti-Mouse CD8 β (H35-17.2, BD), APC anti-mouse CD25 Antibody (PC61, Biolegend), PerCP-Cy5.5 Rat Anti-Mouse CD44 (IM7, BD), APC Rat Anti-Mouse CD44 (IM7, BD), APC-R700 Rat Anti-Mouse CD44 (IM7, BD), eFluor 450 CD45.1 Monoclonal Antibody (A20, Fisher Scientific), PE Mouse Anti-Mouse CD45.1 (A20, BD), APC-Cy7 CD45.1 (A20, BD), PE Mouse Anti-Mouse CD45.2 (104, BD), APC Mouse anti-Mouse CD45.2 (104, BD), BV605 Hamster Anti-Rat/Mouse CD49a (Ha31/8, BD), BV786 Rat Anti-Mouse CD49d (R1-2, BD), PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), PE-Cy7 Rat Anti-Mouse CD62L (MEL-14, BD), APC Rat Anti-Mouse CD62L (MEL-14, BD), APC/Cyanine7 anti-mouse CD62L Antibody (MEL-14, Biolegend), PerCP-Cy5.5 Hamster Anti-Mouse CD69 (H1.2F3, BD), PE-Cy7 Hamster Anti-Mouse CD69 (H1.2F3, BD), Brilliant Violet 605™ anti-mouse CD73 Antibody (TY/11.8, Biolegend), eFluor 450 CD103 Monoclonal Antibody (2E7, Fisher Scientific), eF660 CD127 (A7R34, eBiosciences), Brilliant Violet 711 anti-mouse CX3CR1 (SA011F11, Biolegend), BV711 CXCR3 (CXCR3-173, BD), Brilliant Violet 650™ anti-mouse Ly-6C Antibody (HK1.4, Biolegend), FITC anti-mouse Ly-6C (HK1.4, Biolegend), APC Ly-6C Monoclonal Antibody (HK1.4, eBiosciences), PE-CF594 Hamster Anti-Mouse KLRG1 (2F1, BD), BUV737 Hamster Anti-Mouse TCR β Chain (H57-597, BD), Alexa-Fluor®488 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-Cy™7 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-CF594 TCR β Chain (H57-597, BD), eFluor 450 TRCR V alpha 2 (B20.1, Invitrogen), PerCP/Cy5.5 anti-mouse TCR Vα2 (B20.1, Biolegend).

Techniques: Expressing, Flow Cytometry, Staining, MANN-WHITNEY

A. Scheme of the experiment. B. Representative dot plots of WT (CD45.1-) and SUV39H1 KO (CD45.1+) OTI cells (Vα2+) in the indicated organs 33 days after adoptive transfer. C. Quantification of the ratio between SUV39H1 KO and WT OTI cells within each organ. D. Representative dot plot of intravascular (iv) staining ( left ) and expression of CD49d and Ly6c ( right ) in each population at day 34 post transfer in CD8 + T cells from lungs from Exp. 2 in C . E. Quantification of the percentage and absolute numbers of CD49d + Ly6c - cells among SUV39H1KO or WT OTI cells in the indicated organs from Exp 1 in C . Numbers represent the fold increase between KO and WT condition for each organ. F. Histogram of CD69 expression in iv-CD49d + Ly6c - CD8 + T cells and proportions of CD69+ cells among iv+ and iv-CD49d + Ly6c - CD8 + T cells in lungs from Exp. 4 in C . Numbers represent p values. Each dot represents a mouse, different shapes represent different experiments. * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by one sample t test compared to 1 ( C ), multiple ratio paired t test with Holm-Šídák correction ( E ) and multiple paired t test ( F ).

Journal: bioRxiv

Article Title: Epigenetic control of CD8 + T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

doi: 10.1101/2024.05.13.593994

Figure Lengend Snippet: A. Scheme of the experiment. B. Representative dot plots of WT (CD45.1-) and SUV39H1 KO (CD45.1+) OTI cells (Vα2+) in the indicated organs 33 days after adoptive transfer. C. Quantification of the ratio between SUV39H1 KO and WT OTI cells within each organ. D. Representative dot plot of intravascular (iv) staining ( left ) and expression of CD49d and Ly6c ( right ) in each population at day 34 post transfer in CD8 + T cells from lungs from Exp. 2 in C . E. Quantification of the percentage and absolute numbers of CD49d + Ly6c - cells among SUV39H1KO or WT OTI cells in the indicated organs from Exp 1 in C . Numbers represent the fold increase between KO and WT condition for each organ. F. Histogram of CD69 expression in iv-CD49d + Ly6c - CD8 + T cells and proportions of CD69+ cells among iv+ and iv-CD49d + Ly6c - CD8 + T cells in lungs from Exp. 4 in C . Numbers represent p values. Each dot represents a mouse, different shapes represent different experiments. * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by one sample t test compared to 1 ( C ), multiple ratio paired t test with Holm-Šídák correction ( E ) and multiple paired t test ( F ).

Article Snippet: Cell suspensions were prepared in PBS and stained with Near IR (Fisher Scientific L34975) or Acqua LIVE/DEAD™ Fixable Cell Stain Kit (Fisher Scientific L34957) according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3, eBiosciences), FITC anti-CD8α (53-6.7, BD), PerCP-Cy5.5 CD8 α (53-6.7, BD), PE CD8 α Monoclonal Antibody (53-6.7, Ficher Scientific), BUV395 Rat Anti-Mouse CD8 β (H35-17.2, BD), APC anti-mouse CD25 Antibody (PC61, Biolegend), PerCP-Cy5.5 Rat Anti-Mouse CD44 (IM7, BD), APC Rat Anti-Mouse CD44 (IM7, BD), APC-R700 Rat Anti-Mouse CD44 (IM7, BD), eFluor 450 CD45.1 Monoclonal Antibody (A20, Fisher Scientific), PE Mouse Anti-Mouse CD45.1 (A20, BD), APC-Cy7 CD45.1 (A20, BD), PE Mouse Anti-Mouse CD45.2 (104, BD), APC Mouse anti-Mouse CD45.2 (104, BD), BV605 Hamster Anti-Rat/Mouse CD49a (Ha31/8, BD), BV786 Rat Anti-Mouse CD49d (R1-2, BD), PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), PE-Cy7 Rat Anti-Mouse CD62L (MEL-14, BD), APC Rat Anti-Mouse CD62L (MEL-14, BD), APC/Cyanine7 anti-mouse CD62L Antibody (MEL-14, Biolegend), PerCP-Cy5.5 Hamster Anti-Mouse CD69 (H1.2F3, BD), PE-Cy7 Hamster Anti-Mouse CD69 (H1.2F3, BD), Brilliant Violet 605™ anti-mouse CD73 Antibody (TY/11.8, Biolegend), eFluor 450 CD103 Monoclonal Antibody (2E7, Fisher Scientific), eF660 CD127 (A7R34, eBiosciences), Brilliant Violet 711 anti-mouse CX3CR1 (SA011F11, Biolegend), BV711 CXCR3 (CXCR3-173, BD), Brilliant Violet 650™ anti-mouse Ly-6C Antibody (HK1.4, Biolegend), FITC anti-mouse Ly-6C (HK1.4, Biolegend), APC Ly-6C Monoclonal Antibody (HK1.4, eBiosciences), PE-CF594 Hamster Anti-Mouse KLRG1 (2F1, BD), BUV737 Hamster Anti-Mouse TCR β Chain (H57-597, BD), Alexa-Fluor®488 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-Cy™7 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-CF594 TCR β Chain (H57-597, BD), eFluor 450 TRCR V alpha 2 (B20.1, Invitrogen), PerCP/Cy5.5 anti-mouse TCR Vα2 (B20.1, Biolegend).

Techniques: Adoptive Transfer Assay, Staining, Expressing

A. Scheme of the experiment. B. Representative dot plots and quantification of the normalized numbers of iv-CD44 + CD8 + T cells and the normalized percentages of CD49d + Ly6c - cells among iv-CD44 + CD8 + T cells in lungs 28 days post X31 infection ( Memory ). Values were normalized to the mean of X31-infected WT group for each experiment and then pooled. C. Representative dot plots and quantification of the number of iv-CD44 + CD8 + T cells in lungs 3 days post PR8 infection ( Recall ). D. Representative dot plots and quantification of the number of CD49d + Ly6c - and CD69 + CD103 - iv-CD44 + CD8 + T cells in lungs 3 days post PR8 infection ( Recall ). * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; ns: not significant by ANOVA test with Šídák’s multiple comparisons test.

Journal: bioRxiv

Article Title: Epigenetic control of CD8 + T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

doi: 10.1101/2024.05.13.593994

Figure Lengend Snippet: A. Scheme of the experiment. B. Representative dot plots and quantification of the normalized numbers of iv-CD44 + CD8 + T cells and the normalized percentages of CD49d + Ly6c - cells among iv-CD44 + CD8 + T cells in lungs 28 days post X31 infection ( Memory ). Values were normalized to the mean of X31-infected WT group for each experiment and then pooled. C. Representative dot plots and quantification of the number of iv-CD44 + CD8 + T cells in lungs 3 days post PR8 infection ( Recall ). D. Representative dot plots and quantification of the number of CD49d + Ly6c - and CD69 + CD103 - iv-CD44 + CD8 + T cells in lungs 3 days post PR8 infection ( Recall ). * p<0.05 ; ** p<0.01 ; *** p< 0.001 ; ns: not significant by ANOVA test with Šídák’s multiple comparisons test.

Article Snippet: Cell suspensions were prepared in PBS and stained with Near IR (Fisher Scientific L34975) or Acqua LIVE/DEAD™ Fixable Cell Stain Kit (Fisher Scientific L34957) according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3, eBiosciences), FITC anti-CD8α (53-6.7, BD), PerCP-Cy5.5 CD8 α (53-6.7, BD), PE CD8 α Monoclonal Antibody (53-6.7, Ficher Scientific), BUV395 Rat Anti-Mouse CD8 β (H35-17.2, BD), APC anti-mouse CD25 Antibody (PC61, Biolegend), PerCP-Cy5.5 Rat Anti-Mouse CD44 (IM7, BD), APC Rat Anti-Mouse CD44 (IM7, BD), APC-R700 Rat Anti-Mouse CD44 (IM7, BD), eFluor 450 CD45.1 Monoclonal Antibody (A20, Fisher Scientific), PE Mouse Anti-Mouse CD45.1 (A20, BD), APC-Cy7 CD45.1 (A20, BD), PE Mouse Anti-Mouse CD45.2 (104, BD), APC Mouse anti-Mouse CD45.2 (104, BD), BV605 Hamster Anti-Rat/Mouse CD49a (Ha31/8, BD), BV786 Rat Anti-Mouse CD49d (R1-2, BD), PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), PE-Cy7 Rat Anti-Mouse CD62L (MEL-14, BD), APC Rat Anti-Mouse CD62L (MEL-14, BD), APC/Cyanine7 anti-mouse CD62L Antibody (MEL-14, Biolegend), PerCP-Cy5.5 Hamster Anti-Mouse CD69 (H1.2F3, BD), PE-Cy7 Hamster Anti-Mouse CD69 (H1.2F3, BD), Brilliant Violet 605™ anti-mouse CD73 Antibody (TY/11.8, Biolegend), eFluor 450 CD103 Monoclonal Antibody (2E7, Fisher Scientific), eF660 CD127 (A7R34, eBiosciences), Brilliant Violet 711 anti-mouse CX3CR1 (SA011F11, Biolegend), BV711 CXCR3 (CXCR3-173, BD), Brilliant Violet 650™ anti-mouse Ly-6C Antibody (HK1.4, Biolegend), FITC anti-mouse Ly-6C (HK1.4, Biolegend), APC Ly-6C Monoclonal Antibody (HK1.4, eBiosciences), PE-CF594 Hamster Anti-Mouse KLRG1 (2F1, BD), BUV737 Hamster Anti-Mouse TCR β Chain (H57-597, BD), Alexa-Fluor®488 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-Cy™7 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-CF594 TCR β Chain (H57-597, BD), eFluor 450 TRCR V alpha 2 (B20.1, Invitrogen), PerCP/Cy5.5 anti-mouse TCR Vα2 (B20.1, Biolegend).

Techniques: Infection

A. Scheme of the experiment. B. Representative dot plots and quantification of the expression of CD69 and CD103 in SUV39H1-deficient and WT OTI cells 30 days post X31-OVA infection in lungs ( Memory ). C. Representative dot plots from lungs and quantification of the percentage of CD49d + Ly6c - cells among SUV39H1-deficient and WT OTI cells in the indicated organs and the percentage of CD69 + KLRG1 - or CD49a + CX3CR1 - cells among CD49d + Ly6c - iv-CD44 + OTI cells in lungs 9 days post X31-OVA infection ( Acute ). D. Scheme of the experiment. E. Gating strategy, representative dot plots and quantification of the percentage and numbers of WT and SUV39H1 KO OTI cells among CD69 + CD103 - and CD69 + CD103 + iv-CD44 + OTI cells in lungs 28 days post X31-OVA infection ( Memory ) and 27 days post adoptive transfer. p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by multiple ratio paired t test (B), (C) top and (E) and ratio pared t test (C) bottom. B, C and E : Representative data from 2 independent experiments.

Journal: bioRxiv

Article Title: Epigenetic control of CD8 + T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

doi: 10.1101/2024.05.13.593994

Figure Lengend Snippet: A. Scheme of the experiment. B. Representative dot plots and quantification of the expression of CD69 and CD103 in SUV39H1-deficient and WT OTI cells 30 days post X31-OVA infection in lungs ( Memory ). C. Representative dot plots from lungs and quantification of the percentage of CD49d + Ly6c - cells among SUV39H1-deficient and WT OTI cells in the indicated organs and the percentage of CD69 + KLRG1 - or CD49a + CX3CR1 - cells among CD49d + Ly6c - iv-CD44 + OTI cells in lungs 9 days post X31-OVA infection ( Acute ). D. Scheme of the experiment. E. Gating strategy, representative dot plots and quantification of the percentage and numbers of WT and SUV39H1 KO OTI cells among CD69 + CD103 - and CD69 + CD103 + iv-CD44 + OTI cells in lungs 28 days post X31-OVA infection ( Memory ) and 27 days post adoptive transfer. p<0.05 ; ** p<0.01 ; *** p< 0.001 ; **** p<0.0001 ; ns: not significant by multiple ratio paired t test (B), (C) top and (E) and ratio pared t test (C) bottom. B, C and E : Representative data from 2 independent experiments.

Article Snippet: Cell suspensions were prepared in PBS and stained with Near IR (Fisher Scientific L34975) or Acqua LIVE/DEAD™ Fixable Cell Stain Kit (Fisher Scientific L34957) according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3, eBiosciences), FITC anti-CD8α (53-6.7, BD), PerCP-Cy5.5 CD8 α (53-6.7, BD), PE CD8 α Monoclonal Antibody (53-6.7, Ficher Scientific), BUV395 Rat Anti-Mouse CD8 β (H35-17.2, BD), APC anti-mouse CD25 Antibody (PC61, Biolegend), PerCP-Cy5.5 Rat Anti-Mouse CD44 (IM7, BD), APC Rat Anti-Mouse CD44 (IM7, BD), APC-R700 Rat Anti-Mouse CD44 (IM7, BD), eFluor 450 CD45.1 Monoclonal Antibody (A20, Fisher Scientific), PE Mouse Anti-Mouse CD45.1 (A20, BD), APC-Cy7 CD45.1 (A20, BD), PE Mouse Anti-Mouse CD45.2 (104, BD), APC Mouse anti-Mouse CD45.2 (104, BD), BV605 Hamster Anti-Rat/Mouse CD49a (Ha31/8, BD), BV786 Rat Anti-Mouse CD49d (R1-2, BD), PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), PE-Cy7 Rat Anti-Mouse CD62L (MEL-14, BD), APC Rat Anti-Mouse CD62L (MEL-14, BD), APC/Cyanine7 anti-mouse CD62L Antibody (MEL-14, Biolegend), PerCP-Cy5.5 Hamster Anti-Mouse CD69 (H1.2F3, BD), PE-Cy7 Hamster Anti-Mouse CD69 (H1.2F3, BD), Brilliant Violet 605™ anti-mouse CD73 Antibody (TY/11.8, Biolegend), eFluor 450 CD103 Monoclonal Antibody (2E7, Fisher Scientific), eF660 CD127 (A7R34, eBiosciences), Brilliant Violet 711 anti-mouse CX3CR1 (SA011F11, Biolegend), BV711 CXCR3 (CXCR3-173, BD), Brilliant Violet 650™ anti-mouse Ly-6C Antibody (HK1.4, Biolegend), FITC anti-mouse Ly-6C (HK1.4, Biolegend), APC Ly-6C Monoclonal Antibody (HK1.4, eBiosciences), PE-CF594 Hamster Anti-Mouse KLRG1 (2F1, BD), BUV737 Hamster Anti-Mouse TCR β Chain (H57-597, BD), Alexa-Fluor®488 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-Cy™7 Hamster Anti-Mouse TCR β Chain (H57-597, BD), PE-CF594 TCR β Chain (H57-597, BD), eFluor 450 TRCR V alpha 2 (B20.1, Invitrogen), PerCP/Cy5.5 anti-mouse TCR Vα2 (B20.1, Biolegend).

Techniques: Expressing, Infection, Adoptive Transfer Assay